Application
ICC/IF analysis of methanol-fixed MEF cells using GSK3 alpha/beta (phospho Tyr279/216) antibody.Application
Immunofluorescence staining of methanol-fixed Hela cells using GSK3 beta (Phospho-Tyr216) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 beta (Phospho-Tyr216) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3 beta (Phospho-Tyr216) Antibody .Application
Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3α/β (Phospho-Tyr279/216) Antibody| Product Name | GSK3 alpha/beta (phospho Tyr279/216) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GSK3 alpha/beta (phospho Tyr279/216)This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease. [provided by RefSeq, Oct 2011] |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 279/216 (V-S-Y(p)-I-C) derived from Human GSK3α/β. |
| Modification | p-Tyr279/216 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200. The application notes include recommended starting dilutions optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSK3 alpha phospho Tyr279/216 Phospho GSK3 alpha (pY279/216) phospho GSK3 alpha Tyr279/216 phospho GSK3 alpha Y279/216 GSK3A phospho Y279/216 GSK3 alpha phospho Tyr279/216 GSK3 alpha GSK3 alpha Tyr279/216 phospho phospho GSK3 alpha |
|---|---|
| Target | GSK3 alpha/beta (phospho Tyr279/216) |
| Molecular Weight(MW) | 46, 51 kDa (note) |
| Tissue Specificity | The antibody detects endogenous level of GSK3α/β only when phosphorylated at tyrosine 279/216. |
Application
ICC/IF analysis of methanol-fixed MEF cells using GSK3 alpha/beta (phospho Tyr279/216) antibody.
Application
Immunofluorescence staining of methanol-fixed Hela cells using GSK3 beta (Phospho-Tyr216) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 beta (Phospho-Tyr216) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3 beta (Phospho-Tyr216) Antibody .
Application
Western blot analysis of extracts from 293 cells untreated or treated with PMA using GSK3α/β (Phospho-Tyr279/216) Antibody| Application Notes | Western blotting: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200. The application notes include recommended starting dilutions optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C for long term preservation (recommended). Store at 4°C for short term use. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
