Application
IHC-P analysis of human lung carcinoma using GSK3 beta (phospho Ser9) antibody.Application
ICC/IF analysis of methanol-fixed HeLa cells using using GSK3 beta (phospho Ser9) antibody (green), Phalloidin (red) and DAPI (blue).Application
WB analysis of extracts from HeLa cells treated with EGF or calf intestinal phosphatase (CIP) using GSK3 beta (phospho Ser9) antibody.Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3 beta (Phospho-Ser9) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 beta (Phospho-Ser9) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GSK3 beta (Phospho-Ser9) Antibody .| Product Name | GSK3 beta (phospho Ser9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSK3 beta (Phospho Ser9)Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. |
| Immunogen | Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3 beta . |
| Modification | p-Ser9 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSK3B GSK-3 beta serine/threonine-protein kinase GSK3B glycogen synthase kinase-3 beta GSK3beta isoform glycogen synthase kinase 3 beta |
|---|---|
| Target | GSK3 beta (Phospho Ser9) |
| Molecular Weight(MW) | 46 (note) |
Application
IHC-P analysis of human lung carcinoma using GSK3 beta (phospho Ser9) antibody.
Application
ICC/IF analysis of methanol-fixed HeLa cells using using GSK3 beta (phospho Ser9) antibody (green), Phalloidin (red) and DAPI (blue).
Application
WB analysis of extracts from HeLa cells treated with EGF or calf intestinal phosphatase (CIP) using GSK3 beta (phospho Ser9) antibody.
Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3 beta (Phospho-Ser9) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 beta (Phospho-Ser9) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from 293 cells untreated (lane 1) or treated with serum (lane 2) using GSK3 beta (Phospho-Ser9) Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
