Application
Rabbit anti-GSK3B pS9 was used at a 1:200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded.Application
Western blot using GeneTex Immunochemical's Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kDa. Cells were serum starved for 24 h prior to extraction. Lane 1: Control, Lane 2: treated with IGF-1 (100 ng/ml) for 20' , lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'. Molecular weight markers confirm a MW of ~ 49 kDa.| Product Name | GSK3 beta (phospho Ser9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSK3 beta (phospho Ser9)Glycogen synthase kinase-3 (GSK 3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase. Two isoforms, alpha (GSK 3A) and beta, show a high degree of amino acid homology. GSK 3B is involved in energy metabolism, neuronal cell development, and body pattern formation. |
| Immunogen | Synthetic peptide: RRTTpSFAE, corresponding to amino acids 4-12 of Human GSK3 beta. |
| Modification | p-Ser9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanDrosophila |
| Predicted Reactivity | drosophiladrosophila(88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GSK3B |
|---|---|
| Target | GSK3 beta |
Application
Rabbit anti-GSK3B pS9 was used at a 1:200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue. Tissue was formalin-fixed and paraffin embedded.
Application
Western blot using GeneTex Immunochemical's Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kDa. Cells were serum starved for 24 h prior to extraction. Lane 1: Control, Lane 2: treated with IGF-1 (100 ng/ml) for 20' , lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'. Molecular weight markers confirm a MW of ~ 49 kDa.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
