IHC
Tan IIA increased the expression of p-MEK (A), p-ERK1/2 (B) and p-GSK-3β (C), as assessed by immunohistochemical analysis.WB
Tan IIA increases the expression of p-MEK (A), p-ERK1/2 (B) and p-GSK-3β (C), as detected by immunoblotting.WB
(E) Tan IIA increased the expression of p-MEK, p-ERK1/2 and p-GSK-3β, as detected by immunoblotting. G15 could diminish the expression of these proteins induced by Tan IIA. Previous| Product Name | GSK3 beta (phospho Ser9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to GSK3 beta (phospho Ser9)Glycogen synthase kinase 3 (GSK-3) is a serine/threonine protein kinase that has been implicated in the regulation of cell fate and in the Wnt signaling pathway. GSK-3 plays an important role in the PI3 kinase and Akt mediated cell survival pathways, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3alpha and Ser9 of GSK-3beta. GSK-3 has also been implicated in alzheimer’s disease. Six Tau protein isoforms have been identified, all of which are phosphorylated by GSK-3. |
| Immunogen | Synthetic peptide surrounding Ser9 of human GSK-3β |
| Modification | p-Ser9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western blot analysis (1-2 µg/ml) and Immunohistochemistry (15-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated GSK-3: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | glycogen synthase kinase 3 beta phosphoGSK3b (pSer9) phospho-GSK-3b (pSer9) phospho GSK-3b (pSer9) phospho-GSK-3b (pS9) GSK-3beta EC 2 glycogen synthase kinase3 beta EC2 GSK3beta GSK3B phospho GSK3b (pS9) phosphoGSK3b (pS9) phospho GSK-3b (pS9 |
|---|---|
| Target | GSK3 beta (phospho Ser9) |
| Tissue Specificity | This antibody is specific for GSK-3b (phospho Ser9). |
IHC
Tan IIA increased the expression of p-MEK (A), p-ERK1/2 (B) and p-GSK-3β (C), as assessed by immunohistochemical analysis.
WB
Tan IIA increases the expression of p-MEK (A), p-ERK1/2 (B) and p-GSK-3β (C), as detected by immunoblotting.
WB
(E) Tan IIA increased the expression of p-MEK, p-ERK1/2 and p-GSK-3β, as detected by immunoblotting. G15 could diminish the expression of these proteins induced by Tan IIA. Previous| Application Notes | Western blot analysis (1-2 µg/ml) and Immunohistochemistry (15-30 µg/ml). However, the optimal conditions should be determined individually. The antibody detects phosphorylated GSK-3: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Store at +4C. Product should remain stable for at least 12 months. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% thimerosal |
