Application
Western blot analysis of extracts from A2780 and HT29 cells using GSK3 beta Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic and nuclear staining using GSK3 beta Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 beta Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from A2780 and HT29 cells using GSK3b(Ab-9) Antibody| Product Name | GSK3 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSK3 betaParticipates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. |
| Immunogen | Peptide sequence around aa.7~11 (T-T-S-F-A) derived from Human GSK3 beta . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GSK3B GSK-3 beta serine/threonine-protein kinase GSK3B glycogen synthase kinase-3 beta GSK3beta isoform glycogen synthase kinase 3 beta |
|---|---|
| Target | GSK3 beta |
| Molecular Weight(MW) | 46 (note) |
Application
Western blot analysis of extracts from A2780 and HT29 cells using GSK3 beta Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic and nuclear staining using GSK3 beta Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3 beta Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from A2780 and HT29 cells using GSK3b(Ab-9) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
