Application
GSTA1 antibody detects GSTA1 protein by Western blot analysis. A. 10 µg mouse liver lysate/extract 12 % SDS-PAGE GSTA1 antibody dilution: 1:10000Application
Immunofluorescence analysis of methanol-fixed Hep3B, using GSTA1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using GSTA1 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000| Product Name | GSTA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GSTA1 (glutathione S-transferase alpha 1)Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GSTA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Pig, Sheep, Rhesus MonkeyPig (85%), Sheep (82%), Rhesus Monkey (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GST2 antibody GSTA1-1 antibody GTH1 antibody MGC131939 antibody GSTA1 antibody "GST class alpha 1 antibody" GST HA subunit 1 antibody glutathione S-aryltransferase A1 antibody glutathione S-transferase Ha subunit 1 antibody GST class-alpha membe |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
GSTA1 antibody detects GSTA1 protein by Western blot analysis. A. 10 µg mouse liver lysate/extract 12 % SDS-PAGE GSTA1 antibody dilution: 1:10000
Application
Immunofluorescence analysis of methanol-fixed Hep3B, using GSTA1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using GSTA1 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000| Positive Control | HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
