Application
Sample (30 ug of whole cell lysate) A: 293T B: HeLa 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GTF2A1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using GTF2A1 antibody at 1:500 dilution.| Product Name | GTF2A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to GTF2A1 (transcription initiation factor IIA subunit 1)Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human GTF2A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (96%), Rat (98%), Xenopus laevis (84%), Chicken (93%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC129969 antibody MGC129970 antibody TF2A1 antibody TFIIA antibody GTF2A1 antibody transcription initiation factor IIA subunit 1 antibody TFIIA-42 antibody general transcription factor IIA subunit 1 antibody "glucose regulated protein 58kD pseudo |
|---|---|
| Molecular Weight(MW) | 42 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: 293T B: HeLa 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GTF2A1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using GTF2A1 antibody at 1:500 dilution.| Positive Control | 293T , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
