Application
H6PD antibody [N2N3] detects H6PD protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. H6PD antibody [N2N3] dilution: 1:500.Application
Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1000Application
Sample (50 ug of whole cell lysate) A: Mouse liver 7.5% SDS PAGE diluted at 1:1000| Product Name | H6PD antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to H6PD (hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase))There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human H6PD. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (85%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686A01246 antibody G6PDH antibody GDH antibody MGC87643 antibody H6PD antibody "glucose-6-phosphate dehydrogenase salivary antibody" glucose 1- dehydrogenase antibody 6-phosphogluconolactonase antibody glucose dehydrogenase antibody glucose |
|---|---|
| Molecular Weight(MW) | 89 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum lumen |
Application
H6PD antibody [N2N3] detects H6PD protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. H6PD antibody [N2N3] dilution: 1:500.
Application
Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
Sample (50 ug of whole cell lysate) A: Mouse liver 7.5% SDS PAGE diluted at 1:1000| Positive Control | H1299 , HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
