Application
Immunocytochemistry/Immunofluorescence: HA Epitope Tag Antibody - CHO cellApplication
Immunoprecipitation: HA Epitope Tag Antibody - Immunoprecipitation: HA Epitope Tag Antibody- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.Application
Western Blot: HA Epitope Tag Antibody - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).Application
Western Blot: HA Epitope Tag Antibody - Western Blot: HA Epitope Tag Antibody-Samples: Extracts from S. Cerevisae co-transformed with plasmids expressing HA Tag G alpha and HA tagged RGS domain of RGS beta were screened by Western Blot to identify clones expressing both proteins. Antibody: affinity purifed rabbit anti-HA used at 0.1 mcg/mlApplication
Western Blot: HA Epitope Tag Antibody - Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multitag fusion protein. Affinity purified rabbit anti-HA antibody used for WB at 0.04 ug/ml (1:25,000).Application
Western blot detection of 1 ug of recombinant E. coli protein (containing 11 different epitopes) using Lane 1 contains the antibody (1:1000) alone, lane 2 contains the antibody and neutralizing peptide.| Product Name | HA tag antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Product description | Rabbit polyclonal to HA tagThe HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors. |
| Immunogen | YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIPWB |
| Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | HA epitope tag HA tag HA |
|---|---|
| Target | HA tag |
| Tissue Specificity | ELISA: The anti HA diluted 1:70,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification. HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate. |
| Cellular Localization | various |
Application
Immunocytochemistry/Immunofluorescence: HA Epitope Tag Antibody - CHO cell
Application
Immunoprecipitation: HA Epitope Tag Antibody - Immunoprecipitation: HA Epitope Tag Antibody- Samples: Lysate(150 mcg) from cells that were untransfected or stably transfected with a construct encoding an HA-tagged HIF 1-alpha truncation mutant. Affinity purified rabbit anti-HA used at 3mcg -150mcg lysate. Immunoprecipitated HA-HIF-1 alpha was detected using a monoclonal antibody to HIF-1 alpha.
Application
Western Blot: HA Epitope Tag Antibody - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).
Application
Western Blot: HA Epitope Tag Antibody - Western Blot: HA Epitope Tag Antibody-Samples: Extracts from S. Cerevisae co-transformed with plasmids expressing HA Tag G alpha and HA tagged RGS domain of RGS beta were screened by Western Blot to identify clones expressing both proteins. Antibody: affinity purifed rabbit anti-HA used at 0.1 mcg/ml
Application
Western Blot: HA Epitope Tag Antibody - Detection of HA-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multitag fusion protein. Affinity purified rabbit anti-HA antibody used for WB at 0.04 ug/ml (1:25,000).
Application
Western blot detection of 1 ug of recombinant E. coli protein (containing 11 different epitopes) using Lane 1 contains the antibody (1:1000) alone, lane 2 contains the antibody and neutralizing peptide.| Application Notes | Suggested starting dilutions are as follows: WB: 1:1000-1:10000, ICC/IF: 1:100-1:1000, IP: 1:100-1:500, ELISA. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
