Application
Sample (50 ug of whole cell lysate) A: Mouse liver 12% SDS PAGE diluted at 1:10000Application
Sample(30 μg of whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:500| Product Name | HAAO antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HAAO (3-hydroxyanthranilate 3,4-dioxygenase)3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HAAO. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, BovineRat (90%), Pig (88%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 3-HAO antibody HAO antibody HAAO antibody HAD antibody "3-hydroxyanthranilate 3 4-dioxygenase antibody" 3-hydroxyanthranilate oxygenase antibody 3-hydroxyanthranilic acid dioxygenase antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: Mouse liver 12% SDS PAGE diluted at 1:10000
Application
Sample(30 μg of whole cell lysate) A:Hep G2 12% SDS PAGE diluted at 1:500| Positive Control | HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
