Application
Haptoglobin antibody detects Haptoglobin protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract C. 30 µg A375 whole cell lysate/extract 10% SDS-PAGE Haptoglobin antibody dilution: 1:500Application
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Haptoglobin antibody diluted at 1:5000.| Product Name | Haptoglobin antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Haptoglobin (haptoglobin)This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Haptoglobin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cat, Pig, Rabbit, Rhesus Monkey, ChimpanzeeMouse (83%), Rat (80%), Cat (84%), Pig (82%), Rabbit (87%), Rhesus Monkey (96%), Chimpanzee (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BP antibody HP2ALPHA2 antibody HPA1S antibody MGC111141 antibody HP antibody haptoglobin antibody haptoglobin alpha polypeptide antibody haptoglobin beta polypeptide antibody binding peptide antibody haptoglobin alpha(2FS)-beta antibody haptogl |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (3) |
| Cellular Localization | Secreted |
Application
Haptoglobin antibody detects Haptoglobin protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HepG2 whole cell lysate/extract C. 30 µg A375 whole cell lysate/extract 10% SDS-PAGE Haptoglobin antibody dilution: 1:500
Application
Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Haptoglobin antibody diluted at 1:5000.| Positive Control | HeLa , HepG2 , A375 , human plasma |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
