Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using HARS antibody at 1:500 dilution.Application
HARS antibody [N1C1] detects HARS protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract B. 30 µg HCT116 whole cell lysate/extract 7.5 % SDS-PAGE HARS antibody [N1C1] dilution: 1:500| Product Name | HARS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HARS (histidyl-tRNA synthetase)Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HARS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Chicken, Chimpanzee, BovineMouse (96%), Rat (96%), Zebrafish (84%), Chicken (89%), Chimpanzee (99%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20491 antibody HRS antibody HARS antibody "histidine tRNA ligase 1 cytoplasmic antibody" histidine-tRNA ligase antibody HisRS antibody "histidyl-tRNA synthetase cytoplasmic antibody" histidine--tRNA ligase antibody histidine translase antibody |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (5) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using HARS antibody at 1:500 dilution.
Application
HARS antibody [N1C1] detects HARS protein by Western blot analysis. A. 30 µg HepG2 whole cell lysate/extract B. 30 µg HCT116 whole cell lysate/extract 7.5 % SDS-PAGE HARS antibody [N1C1] dilution: 1:500| Positive Control | HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
