Application
HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6 , HAUS2 and HAUS1 in U2OS cells.Application
HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000| Product Name | HAUS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HAUS2 (HAUS augmin-like complex, subunit 2)HAUS2 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM] |
| Immunogen | Full length human HAUS2 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (82%), Rhesus Monkey (85%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C15orf25 antibody CEP27 antibody FLJ10460 antibody HsT17025 antibody HAUS2 antibody centrosomal protein of 27 kDa antibody HAUS augmin-like complex subunit 2 antibody centrosomal protein 27kDa antibody "HAUS augmin-like complex subunit 2 antibody" |
|---|---|
| Molecular Weight(MW) | 27 kDa (note) (2) |
| Cellular Localization | Centrosome , Spindle |
Application
HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
IP-WB assay to show that Hice1 co-immunoprecipitated with other Augmin components HAUS6 , HAUS2 and HAUS1 in U2OS cells.
Application
HAUS2 antibody [N1C3-2] detects HAUS2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: HAUS2 protein stained by HAUS2 antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
