Application
Western Blot analysis of phospho and non-phospho Ser76/Ser77| Product Name | Hec1 (phospho Ser 76/77) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Hec1 (phospho Ser 76/77)HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM] |
| Immunogen | Synthetic phosphopeptide (GTX70015-PEP) corresponding to the amino acids around Ser 76/77 conjugated to KLH |
| Modification | p-Ser 76/77 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| For WB: Use at a dilution of 1:300: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HEC hsNDC80 NDC80 homolog kinetochore complex component (S. cerevisiae) NDC80 KNTC2 TID3 HEC3 |
|---|---|
| Target | Hec1 |
| Molecular Weight(MW) | 80 kDa (note) |
| Cellular Localization | Nucleus , Kinetochore |
Application
Western Blot analysis of phospho and non-phospho Ser76/Ser77| Positive Control | HeLa , Blocking peptide (GTX70015-PEP) |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:300: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 1X PBS |
