Application
Sample A: 30ug of A431 whole cell lysate B: 30ug of H1299 whole cell lysate 7.5% SDS PAGE diluted at 1:1000| Product Name | HEC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HEC1 (NDC80 homolog, kinetochore complex component (S. cerevisiae))HEC is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.[supplied by OMIM] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HEC antibody HEC1 antibody KNTC2 antibody TID3 antibody hsNDC80 antibody NDC80 antibody kinetochore protein NDC80 homolog antibody "highly expressed in cancer rich in leucine heptad repeats antibody" kinetochore protein Hec1 antibody "NDC80 homol |
|---|---|
| Target | HEC1 |
| Molecular Weight(MW) | 74 kDa (note) |
| Cellular Localization | Nucleus , Kinetochore |
Application
Sample A: 30ug of A431 whole cell lysate B: 30ug of H1299 whole cell lysate 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
