Application
WB analysis of (1) Jurkat, (2) HeLa, (3) NCCIT and (4) human spleen lysates (10 μg per lane) using HEC1 antibody [EPR5342] at a dilution of 1:10,000.| Product Name | HEC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR5342] to HEC1This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011] |
| Immunogen | A synthetic peptide corresponding to residues near the C-terminus of Human HEC1. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR5342 |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | HEC hsNDC80 NDC80 kinetochore complex component NDC80 HsHec1 KNTC2 TID3 |
|---|---|
| Target | HEC1 |
Application
WB analysis of (1) Jurkat, (2) HeLa, (3) NCCIT and (4) human spleen lysates (10 μg per lane) using HEC1 antibody [EPR5342] at a dilution of 1:10,000.| Application Notes | IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for 12 months at -20°C |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
