Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HELIOS antibody at 1:250 dilution.Application
HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody diluted by 1:500 and developed with Trident femto Western HRP Substrate . The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HELIOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HELIOS (IKAROS family zinc finger 2 (Helios))This gene encodes a member of the Ikaros family of zinc-finger proteins. Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development. This protein forms homo- or hetero-dimers with other Ikaros family members, and is thought to function predominantly in early hematopoietic development. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HELIOS. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Chicken, Rhesus MonkeyMouse (96%), Chicken (87%), Rhesus Monkey (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HELIOS antibody MGC34330 antibody ZNF1A2 antibody ZNFN1A2 antibody IKZF2 antibody zinc finger DNA binding protein Helios antibody ikaros family zinc finger protein 2 antibody "zinc finger protein subfamily 1A 2 (Helios) antibody" zinc finger prot |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HELIOS antibody at 1:250 dilution.
Application
HELIOS antibody detects HELIOS protein by western blot analysis. Jurkat whole cell extracts and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with HELIOS antibody diluted by 1:500 and developed with Trident femto Western HRP Substrate . The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Jurkat NE |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
