Application
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody diluted at 1:500.Application
Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody diluted at 1:500.Application
Untreated (–) and treated (+) mouse tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody diluted at 1:500. (CFA: Complete Freunds adjuvant)WB
MEL cells were transfected with control or lncEry siRNA, and cellular extracts were prepared and analyzed by Western blotting. The intensity of each band was quantified by densitometry with ImageJ software and normalized to β-actin (n = 3 samples).(F) Cellularextracts of NuEcells sorted from BMofflox/flox, Δ/Δ, flox/flox-2, or Δ/Δ-2 mice were preparedand analyzedby Western blotting.| Product Name | Hemoglobin beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Hemoglobin beta (hemoglobin, beta)The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq] |
| Immunogen | Full length human Hemoglobin beta Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (85%), Rhesus Monkey (94%), Bovine (82%) |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | CD113t-C antibody beta-globin antibody HBB antibody hemoglobin beta chain antibody beta globin chain antibody hemoglobin subunit beta antibody "hemoglobin beta antibody" |
|---|---|
| Molecular Weight(MW) | 16 kDa (note) (2) |
Application
Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody diluted at 1:500.
Application
Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody diluted at 1:500.
Application
Untreated (–) and treated (+) mouse tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin beta antibody diluted at 1:500. (CFA: Complete Freunds adjuvant)
WB
MEL cells were transfected with control or lncEry siRNA, and cellular extracts were prepared and analyzed by Western blotting. The intensity of each band was quantified by densitometry with ImageJ software and normalized to β-actin (n = 3 samples).(F) Cellularextracts of NuEcells sorted from BMofflox/flox, Δ/Δ, flox/flox-2, or Δ/Δ-2 mice were preparedand analyzedby Western blotting.| Positive Control | human plasma , Mouse Leukocyte , mouse leukocyte treated with CFA , rat spleen |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
