Application
ErbB2 (phospho Tyr1139) antibody detects ErbB2 (phospho Tyr1139) protein by western blot analysis. Un-treated (-) and treated (+, 200ng/ml Trichostatin A treatment for 15min) A431 whole cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2 (phospho Tyr1139) antibody at a dilution of 1:10000.Application
Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 (phospho Tyr1139) antibody diluted at 1:10000.| Product Name | Her2 / ErbB2 (phospho Tyr1139) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ErbB2 (phospho Tyr1139) (v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian))This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ErbB2 (phospho Tyr1139). The exact sequence is proprietary. |
| Modification | p-Tyr1139 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ErbB2 (pTyr1139) NGL ErbB2 phospho Tyr1139 ErbB2 phospho Y1139 ErbB2 (pY1139) HER-2 HER-2/neu phospho ErbB2 (pY1139) ErbB2 phospho NEU ERBB2 phospho ErbB2 ErbB2 (phospho Y1139) phospho ErbB2 (Y1139) MLN 19 TKR1 |
|---|---|
| Target | ErbB2 (phospho Tyr1139) |
| Molecular Weight(MW) | 138 kDa (note) (3) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
ErbB2 (phospho Tyr1139) antibody detects ErbB2 (phospho Tyr1139) protein by western blot analysis. Un-treated (-) and treated (+, 200ng/ml Trichostatin A treatment for 15min) A431 whole cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2 (phospho Tyr1139) antibody at a dilution of 1:10000.
Application
Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 (phospho Tyr1139) antibody diluted at 1:10000.| Positive Control | A431 ( 200 ng/ml Trichostatin A treatment for 15 min) , A431 (200ng/ml Trichostatin A for 15min) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
