Application
A.Western blot analysis on A) SKBR3 and B) SKBR3 + EGF cell lysate using anti-HER2 / ErbB2 Phospho (pY1139) RabMAb (cat. # ) dilution 1:1000.| Product Name | Her2 / ErbB2 (phospho Tyr1139) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP1046Y] to ErbB2 (phospho Tyr1139)HER2 (ErbB2, epidermal growth factor receptor 2) is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases . The kinase activity of HER2 can be activated without ligand if it is overexpressed, and by association with other HER proteins . Overexpression of HER2 is detected in almost 40% of human breast cancers . Therefore, HER2 is considered one of the major targets for the treatment of breast cancer and other carcinomas. Binding of c-Cbl ubiquitin ligase to Tyr1112 of HER2 leads to poly-ubiquitination of HER2 and enhances its degradation . Similar to other growth factors, HER2 exhibits intrinsic protein tyrosine kinase activity and undergoes autophosphorylation (Y1023, Y1248, Y1139, and Y1222). A phosphorylation at tyrosine 1139 has been identified to induce the binding of Src to HER2, leading to HER2 activation of Stat3 alpha . |
| Immunogen | A synthetic peptide corresponding to residues surrounding tyrosine 1139 of human HER2 / ErbB2 was used as an immunogen. |
| Modification | p-Tyr1139 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP1046Y |
| Host Species | Rabbit |
| Tested Applications | FACSIPWB |
| For WB: Use at a dilution of 1:500-1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MLN19 CD340 HER2 NGL HER2/neu NEU HER-2/neu HER-2 TKR1 MLN 19 ERBB2 HER 2 HER 2/neu HER2 / ErbB2 (phospho Y1139) HER2 / ErbB2 (phospho Tyr1139) HER2 / ErbB2 (pY1139) HER2 / ErbB2 (pTyr1139) HER2 / ErbB2 phospho Y1139 HER2 / ErbB2 phospho |
|---|---|
| Target | HER2 / ErbB2 Phospho (pY1139) |
| Molecular Weight(MW) | 185 (note) |
Application
A.Western blot analysis on A) SKBR3 and B) SKBR3 + EGF cell lysate using anti-HER2 / ErbB2 Phospho (pY1139) RabMAb (cat. # ) dilution 1:1000.| Application Notes | For WB: Use at a dilution of 1:500-1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
