Application
Immunofluorescence staining of methanol-fixed MCF cells using HER2 (Phospho-Tyr1221/Tyr1222) Antibody .Application
Western blot analysis of extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222) Antibody .Application
Western blot analysis of extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222) Antibody| Product Name | Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ErbB2 (Phospho Tyr1221/Tyr1222)Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p)-W) derived from Human HER2. |
| Modification | p-Tyr1221/Tyr1222 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD340 HER-2 HER-2/neu HER2 MLN 19 NEU NGL TKR1 ERBB2 neuroblastoma/glioblastoma derived oncogene homolog v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) herstatin metastatic lymph no |
|---|---|
| Target | HER2 (Phospho Tyr1221/Tyr1222) |
| Molecular Weight(MW) | 185 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF cells using HER2 (Phospho-Tyr1221/Tyr1222) Antibody .
Application
Western blot analysis of extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222) Antibody .
Application
Western blot analysis of extracts from MDA cells untreated or treated with EGF using HER2 (Phospho-Tyr1221/Tyr1222) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
