Application
Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from MDA cells untreated or treated with EGF using HER2 (Phospho Tyr1248) Antibody.Application
WB analysis of extracts from HepG2 cells treated with PMA and MCF cells treated with EGF using ErbB2 (phospho Tyr1248) antibody.Application
IHC-P analysis of human lung carcinoma tissue using ErbB2 (phospho Tyr1248) antibody.| Product Name | Her2 / ErbB2 (phospho Tyr1248) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ErbB2 (Phospho Tyr1248)Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine1248 (P-E-Y(p)-L-G) derived from Human HER2. |
| Modification | p-Tyr1248 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD340 HER-2 HER-2/neu HER2 MLN 19 NEU NGL TKR1 ERBB2 neuroblastoma/glioblastoma derived oncogene homolog v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) herstatin metastatic lymph no |
|---|---|
| Target | HER2 (Phospho Tyr1248) |
| Molecular Weight(MW) | 185 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 (Phospho-Tyr1248) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 (Phospho-Tyr1248) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from MDA cells untreated or treated with EGF using HER2 (Phospho Tyr1248) Antibody.
Application
WB analysis of extracts from HepG2 cells treated with PMA and MCF cells treated with EGF using ErbB2 (phospho Tyr1248) antibody.
Application
IHC-P analysis of human lung carcinoma tissue using ErbB2 (phospho Tyr1248) antibody.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
