Application
Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from MCF and SKOV3 cells using HER2(Ab-1248) Antibody| Product Name | Her2 / ErbB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ErbB2Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin. |
| Immunogen | Peptide sequence around aa. 1246~1250 (P-E-Y-L-G) derived from Human HER2. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Mouse, Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD340 HER-2 HER-2/neu HER2 MLN 19 NEU NGL TKR1 ERBB2 neuroblastoma/glioblastoma derived oncogene homolog v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) herstatin metastatic lymph no |
|---|---|
| Target | HER2 |
| Molecular Weight(MW) | 185 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF7 cells using HER2 Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HER2 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from MCF and SKOV3 cells using HER2(Ab-1248) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
