Application
Human Breast Ductal Carcinoma stained with anti-c-erbB-2/HER-2 antibodyApplication
Formalin fixed paraffin embedded human breast carcinoma stained with c-erbB-2 antibody (cat. # ).Application
Human Breast Carcinoma stained with anti-c-erbB-2/HER-2 antibodyApplication
Human Breast stained with anti-c-erbB-2/HER-2 antibodyApplication
Human Bladder stained with anti-c-erbB-2/HER-2 antibodyApplication
Human Bladder Transitional Cell Carcinoma stained with anti-c-erbB-2/HER-2 antibodyApplication
Human Placenta stained with anti-c-erbB-2/HER-2 antibodyApplication
Human Stomach Carcinoma stained with anti-c-erbB-2/HER-2 antibodyApplication
Human Fallopian Tube stained with anti-c-erbB-2/HER-2 antibodyApplication
Human Prostate Adenocarcinoma stained with anti-c-erbB-2/HER-2 antibody| Product Name | Her2 / ErbB2 antibody (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody to ErbB2This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encoding extracellular domain of human c-erbB-2. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | SP3 |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | c-erb B2 HER2 137215 cerb B2 2064 NEU HER-2/neu cErbB2 NGL HER2/neu P04626 HER-2 TKR1 ERBB2 c ErbB2 CD340 MLN 19 HER 2 c erb B2 HER 2/neu |
|---|---|
| Target | c-ErbB2 |
| Tissue Specificity | This antibody recognizes a c-erbB-2 protein, which is a receptor tyrosine kinase of the c-erbB family. It is closely related in structure to the epidermal growth factor receptor. c-erbB-2 oncoprotein is detectable in a proportion of breast and other adenocarcinomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis. This antibody crossreacts with Human. |
| Cellular Localization | Cell membrane |
Application
Human Breast Ductal Carcinoma stained with anti-c-erbB-2/HER-2 antibody
Application
Formalin fixed paraffin embedded human breast carcinoma stained with c-erbB-2 antibody (cat. # ).
Application
Human Breast Carcinoma stained with anti-c-erbB-2/HER-2 antibody
Application
Human Breast stained with anti-c-erbB-2/HER-2 antibody
Application
Human Bladder stained with anti-c-erbB-2/HER-2 antibody
Application
Human Bladder Transitional Cell Carcinoma stained with anti-c-erbB-2/HER-2 antibody
Application
Human Placenta stained with anti-c-erbB-2/HER-2 antibody
Application
Human Stomach Carcinoma stained with anti-c-erbB-2/HER-2 antibody
Application
Human Fallopian Tube stained with anti-c-erbB-2/HER-2 antibody
Application
Human Prostate Adenocarcinoma stained with anti-c-erbB-2/HER-2 antibody| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | This antibody contains less than 0.1% sodium azide. |
