Application
Confocal IF analysis of MCF7 cells using ErbB3 (phospho Tyr1328) antibody.Application
IHC-P analysis of human breast carcinoma tissue using ErbB3 (phospho Tyr1328) antibody.Application
WB analysis of lysates from untreated and EGF-treated A431 cells using ErbB3 (phospho Tyr1328) antibody.| Product Name | Her3 / ErbB3 (phospho Tyr1328) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ErbB3 (phospho Tyr1328)This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughl |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human Her3/ErbB3 around the phosphorylation site of tyrosine 1328. |
| Modification | p-Tyr1328 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500-1:1000 IHC:1:50-1:100 IF:1:100-1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) c-erbB-3 LCCS2 c-erbB3 ERBB3 p85-sErbB3 p45-sErbB3 ErbB-3 p180-ErbB3 MDA-BF-1 e |
|---|---|
| Target | HER3 |
| Tissue Specificity | This antibody detects endogenous levels of Her3/ErbB3 only when phosphorylated at tyrosine 1328. |
Application
Confocal IF analysis of MCF7 cells using ErbB3 (phospho Tyr1328) antibody.
Application
IHC-P analysis of human breast carcinoma tissue using ErbB3 (phospho Tyr1328) antibody.
Application
WB analysis of lysates from untreated and EGF-treated A431 cells using ErbB3 (phospho Tyr1328) antibody.| Application Notes | WB: 1:500-1:1000 IHC:1:50-1:100 IF:1:100-1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
