Application
IHC-P analysis of human liver tissue using HERC4 antibody at 1:600. Left to right : DAPI, HERC4, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.Application
WB analysis of ACHN cells using HERC4 antibody at 0.2-1μg/ml.| Product Name | HERC4 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HERC4HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human HERC4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 93%; Yeast: 83% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HERC4 |
|---|---|
| Target | HERC4 |
| Molecular Weight(MW) | 119 KDa (note) |
Application
IHC-P analysis of human liver tissue using HERC4 antibody at 1:600. Left to right : DAPI, HERC4, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.
Application
WB analysis of ACHN cells using HERC4 antibody at 0.2-1μg/ml.| Positive Control | ACHN |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
