Application
WB analysis of extracts from various cell lines using HK1 antibody.Application
IHC-P analysis of rat lung tissue using HK1 antibody at a dilution of 1:200.Application
IHC-P analysis of human rectal cancer using HK1 antibody at a dilution of 1:200.Application
IHC-P analysis of human kidney tissue using HK1 antibody at a dilution of 1:200.Application
ICC/IF analysis of U2OS cells using HK1 antibody (green) and DAPI (blue).| Product Name | Hexokinase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Hexokinase 1Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009] |
| Immunogen | Recombinant protein of human HK1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HK1-tb hexokinase 1 HKI HXK1 HK1-ta HK1-tc Hk1 antibody |
|---|---|
| Target | Hexokinase 1 |
| Molecular Weight(MW) | 102 kDa (note) |
Application
WB analysis of extracts from various cell lines using HK1 antibody.
Application
IHC-P analysis of rat lung tissue using HK1 antibody at a dilution of 1:200.
Application
IHC-P analysis of human rectal cancer using HK1 antibody at a dilution of 1:200.
Application
IHC-P analysis of human kidney tissue using HK1 antibody at a dilution of 1:200.
Application
ICC/IF analysis of U2OS cells using HK1 antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
