Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:10000Application
HIBADH antibody detects HIBADH protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE HIBADH antibody dilution: 1:5000Application
HIBADH antibody detects HIBADH protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE HIBADH antibody dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HIBADH antibody at 1:250 dilution.| Product Name | HIBADH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIBADH (3-hydroxyisobutyrate dehydrogenase)3-hydroxyisobutyrate dehydrogenase (3-hydroxy-2-methylpropanoate:NAD(+) oxidoreductase, EC 1.1.1.31) is a dimeric mitochondrial enzyme that catalyzes the NAD(+)-dependent, reversible oxidation of 3-hydroxyisobutyrate, an intermediate of valine catabolism, to methylmalonate semialdehyde.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HIBADH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Chicken, BovineXenopus laevis (85%), Chicken (87%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC40361 antibody NS5ATP1 antibody HIBADH antibody 3'-hydroxyisobutyrate dehydrogenase antibody 3-hydroxyisobutyrate dehydrogenase mitochondrial antibody 3-hydroxyisobutyrate dehydrogenase antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE diluted at 1:10000
Application
HIBADH antibody detects HIBADH protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract 10 % SDS-PAGE HIBADH antibody dilution: 1:5000
Application
HIBADH antibody detects HIBADH protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE HIBADH antibody dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using HIBADH antibody at 1:250 dilution.| Positive Control | A549 , Neuro2A , C8D30 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
