Application
Immunoprecipitation of HIF2 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF2 alpha antibody . Western blot analysis was performed using HIF2 alpha antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody diluted by 1:500.| Product Name | HIF2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIF2 alpha (endothelial PAS domain protein 1)This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HIF2 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, PigMouse (80%), Pig (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | EPAS1 HLF ECYT4 endothelial PAS domain protein 1 HIF2A bHLHe73 MOP2 PASD2 |
|---|---|
| Target | HIF2 alpha |
| Molecular Weight(MW) | 96 kDa (note) |
| Cellular Localization | Nucleus |
Application
Immunoprecipitation of HIF2 alpha protein from HepG2 whole cell extracts treated with 500 μM CoCl2 for 24 hr using 5 μg of HIF2 alpha antibody . Western blot analysis was performed using HIF2 alpha antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
HIF2 alpha antibody detects HIF2 alpha protein by western blot analysis. Un-treated (-) and treated (+, 1% O2 treatment for 24hr) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HIF2 alpha antibody diluted by 1:500.| Positive Control | HepG2 (1% O2 treatment for 24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
