Application
Immunofluorescence analysis of methanol-fixed HeLa, using HIP2 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE HIP2 antibody diluted at 1:1000| Product Name | HIP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIP2 (ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast))The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HIP2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineRat (100%), Xenopus laevis (98%), Pig (100%), Chicken (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp564C1216 antibody DKFZp686J24237 antibody E2-25K antibody HIP2 antibody HYPG antibody LIG antibody UBE2K antibody HIP-2 antibody ubiquitin-conjugating enzyme E2(25K) antibody E2(25K) antibody "ubiquitin-conjugating enzyme E2K (UBC1 homolog |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using HIP2 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE HIP2 antibody diluted at 1:1000| Positive Control | A549 , HeLa , HepG2 , HCT116 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
