Application
Sample (5 ug of whole cell lysate) A: Acid-extracted histones from HeLa cell B: Acid-extracted histones from HeLa cell treated Nocodazole 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H1S35ph (phospho Ser35) antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Histone H1S35ph (phospho Ser35) antibody at 1:500 dilution.| Product Name | Histone H1S35ph (phospho Ser35) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H1.4 (phospho Ser35) (histone cluster 1, H1e)Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Modification | p-Ser35 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H1 histone family antibody Histone H1.4 phospho Ser36 antibody Phospho Histone H1.4 antibody Phospho Histone H1.4 (pSer36) antibody histone H1b antibody H1E antibody H1.4S35ph antibody "histone 1 antibody Histone H1.4 (phospho Ser36) antibody memb |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (5 ug of whole cell lysate) A: Acid-extracted histones from HeLa cell B: Acid-extracted histones from HeLa cell treated Nocodazole 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H1S35ph (phospho Ser35) antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Histone H1S35ph (phospho Ser35) antibody at 1:500 dilution.| Positive Control | Acid-extracted histones from HeLa cell treated Nocodazole |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
