Application
Sample (30 ug of whole cell lysate) A: HCT116 15% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using Histone H2A.x antibody at 1:1000 dilution.Application
Histone H2A.X antibody [N1N2], N-term detects Histone H2A.X protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A.X protein stained by Histone H2A.X antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | Histone H2A.X antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H2A.x (H2A histone family, member X)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2A.X. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Rhesus Monkey, BovineMouse (100%), Rat (100%), Zebrafish (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H2A.X antibody H2A/X antibody H2AX antibody H2AFX antibody H2AX histone antibody histone H2A.x antibody "H2A histone family member X antibody" |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HCT116 15% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using Histone H2A.x antibody at 1:1000 dilution.
Application
Histone H2A.X antibody [N1N2], N-term detects Histone H2A.X protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2A.X protein stained by Histone H2A.X antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HCT116 control |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
