Application
Dot blot analysis of peptides containing different acetylations and the unmodified H2A.Z using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.Application
WB analysis of whole cell extracts (25 μg, lane 1) from HeLa cells, and 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
ICC/IF analysis of HeLa cells using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (green) and DAPI (blue). Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with H2A.Zac antibody at a dilution of 1:500.Application
ChIP was performed with HeLa extracts and either control IgG (2 μg) or Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (1, 2, 5 and 10 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to CCT5 promoter, EIF4A2 promoter, MYT1 gene and the Sat2 satellite repeat.Application
ChIP was performed with K562 extracts and either control IgG (1 μg) or Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (0.2, 0.5, 1 and 2 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to EIF4A2 promoter, MYT1 gene and the Sat2 satellite repeat.Application
ELISA was performed using a serial dilution of Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:56,600.| Product Name | Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to H2A.Z (acetyl Lys5/Lys7/Lys11)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008] |
| Immunogen | Polyclonal antibody raised in rabbit against the region of histone H2A. |
| Modification | acetyl Lys5/Lys7/Lys11 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAICC/IFWBChIP |
| ChIP/ChIP-seq (1 µg/IP), ELISA (1:500), Dot blotting (1:20,000), Western blotting (1:1,000), Immunofluorescence (1:500): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H2A/z H2A.z H2AZ H2A histone family member Z H2AFZ |
|---|---|
| Target | H2A.Z (acetyl Lys5/Lys7/Lys11) |
Application
Dot blot analysis of peptides containing different acetylations and the unmodified H2A.Z using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
Application
WB analysis of whole cell extracts (25 μg, lane 1) from HeLa cells, and 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 2, 3, 4 and 5, respectively) using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
ICC/IF analysis of HeLa cells using Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (green) and DAPI (blue). Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with H2A.Zac antibody at a dilution of 1:500.
Application
ChIP was performed with HeLa extracts and either control IgG (2 μg) or Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (1, 2, 5 and 10 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to CCT5 promoter, EIF4A2 promoter, MYT1 gene and the Sat2 satellite repeat.
Application
ChIP was performed with K562 extracts and either control IgG (1 μg) or Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody (0.2, 0.5, 1 and 2 μg per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to EIF4A2 promoter, MYT1 gene and the Sat2 satellite repeat.
Application
ELISA was performed using a serial dilution of Histone H2A.Z (acetyl Lys5/Lys7/Lys11) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:56,600.| Application Notes | ChIP/ChIP-seq (1 µg/IP), ELISA (1:500), Dot blotting (1:20,000), Western blotting (1:1,000), Immunofluorescence (1:500): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
