Application
RM222 specifically reacts to Histone H2A.Z acetylatedat Lysine 7 (K7ac). No cross reactivity with non-modifiedLysine 7 or other acetylated Lysines in histone H2A.Application
Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate,using RM222 at 0.5 ug/mL, showed a band ofhistone H2A.Z acetylated at Lysine 7 in treatedHeLa.| Product Name | Histone H2A.Z (Acetyl Lys7) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM222] to Histone H2A.Z (Acetyl Lys7)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008] |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H2A.Z (Lys7) |
| Modification | Acetyl Lys7 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM222 |
| Host Species | Rabbit |
| Tested Applications | ELISAWBMultiplexing |
| Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.05 ug/mL – 0.5 ug/mL.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2AZ H2AFZ H2A.z H2A histone family member Z H2A/z |
|---|---|
| Target | Histone H2A.Z (Acetyl Lys7) |
| Tissue Specificity | This antibody reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A. |
Application
RM222 specifically reacts to Histone H2A.Z acetylatedat Lysine 7 (K7ac). No cross reactivity with non-modifiedLysine 7 or other acetylated Lysines in histone H2A.
Application
Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate,using RM222 at 0.5 ug/mL, showed a band ofhistone H2A.Z acetylated at Lysine 7 in treatedHeLa.| Application Notes | Western Blot: 0.5 ug/mL – 2 ug/mL, ELISA: 0.2 ug/mL - 1 ug/mL, Multiplex: 0.05 ug/mL – 0.5 ug/mL.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
