Application
Western Blot of A375, HEK293, HeLa and SK-MEL-2whole cell lysates, using RM215 at 0.5 ug/mL, showedendogenous Histone H2AZ in A375, HEK293, HeLa andSK-MEL-2 cells.| Product Name | Histone H2A.Z antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM215] to Histone H2A.ZHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008] |
| Immunogen | A peptide corresponding to the C-terminus of human Histone H2AZ |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM215 |
| Host Species | Rabbit |
| Tested Applications | ELISAWBMultiplexing |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2A/z H2AFZ H2A.z H2A histone family member Z H2A.Z-1 H2AZ H2A.Z antibody |
|---|---|
| Target | Histone H2A.Z |
| Tissue Specificity | This antibody reacts to Histone H2AZ protein, independent of post-translational modifications. No cross reactivity with other histone proteins. |
Application
Western Blot of A375, HEK293, HeLa and SK-MEL-2whole cell lysates, using RM215 at 0.5 ug/mL, showedendogenous Histone H2AZ in A375, HEK293, HeLa andSK-MEL-2 cells.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
