Application
Histone H2B antibody detects Histone H2B protein at nucleus in mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. Histone H2B antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Histone H2B antibody at 1:500 dilution.Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H2B antibody detects Histone H2B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2B protein stained by Histone H2B antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Histone H2B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HIST1H2BD (histone cluster 1, H2bd)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Immunogen | Full length human Histone H2B Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, BovineRat (98%), Pig (99%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H2B.1B antibody H2B/b antibody H2BFB antibody HIRIP2 antibody MGC90432 antibody dJ221C16.6 antibody HIST1H2BD antibody "histone 1 H2bd antibody" histone H2B.b antibody histone H2B type 1-D antibody HIRA-interacting protein 2 antibody histone H2 |
|---|---|
| Molecular Weight(MW) | 14 kDa (note) (3) |
Application
Histone H2B antibody detects Histone H2B protein at nucleus in mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. Histone H2B antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using Histone H2B antibody at 1:500 dilution.
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2B antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H2B antibody detects Histone H2B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: Histone H2B protein stained by Histone H2B antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
