Application
ChIP was performed with HeLa nuclear extract and either 5 μl of H3pan antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to Sat2 or GAPDH promoter.Application
Dot blot analysis of peptides used for immunization and other peptides containing different modifications or unmodified sequences of histone H3 using Histone H3 antibody at a dilution of 1:1,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.Application
WB analysis of histone extracts (15 μg) from HeLa cells using Histone H3 antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk (lane 1). Lane 2 shows a coomassie blue staining of the gel.Application
ELISA was performed using a serial dilution of Histone H3 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:900,000.| Product Name | Histone H3 antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAWBChIP |
| ChIP (1 - 5 µl/ChIP), ELISA (1:100 – 1:1,000), Dot blotting (1:1,000), Western blotting (1:250): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 1 H3a H3/A HIST1H3A H3FA |
|---|---|
| Target | Histone H3 |
Application
ChIP was performed with HeLa nuclear extract and either 5 μl of H3pan antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to Sat2 or GAPDH promoter.
Application
Dot blot analysis of peptides used for immunization and other peptides containing different modifications or unmodified sequences of histone H3 using Histone H3 antibody at a dilution of 1:1,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
Application
WB analysis of histone extracts (15 μg) from HeLa cells using Histone H3 antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk (lane 1). Lane 2 shows a coomassie blue staining of the gel.
Application
ELISA was performed using a serial dilution of Histone H3 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:900,000.| Application Notes | ChIP (1 - 5 µl/ChIP), ELISA (1:100 – 1:1,000), Dot blotting (1:1,000), Western blotting (1:250): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
