Application
Sample (30 ug of whole cell lysate) A: IMR32 15% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Histone H3.3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3 antibody diluted at 1:500.| Product Name | Histone H3.3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3.3 (H3 histone, family 3A)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Histone H3.3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Pig, Rabbit, BovineMouse (100%), Zebrafish (99%), Xenopus laevis (100%), Pig (100%), Rabbit (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3.3A antibody H3F3 antibody H3F3B antibody MGC87782 antibody MGC87783 antibody H3F3A antibody histone H3.3 antibody "H3 histone family 3A antibody" |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: IMR32 15% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Histone H3.3 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3.3 antibody diluted at 1:500.| Positive Control | IMR32 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
