Application
Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H3K27ac (acetyl Lys27) antibody. The precipitated DNA was detected by PCR with primer set targeting to RPL10 promoter or β-globin promoter.Application
Histone H3 (acetyl K27) antibody immunoprecipitates Histone H3 (acetyl K27) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H3 (acetyl K27) protein expressing HeLa cells (0.4μM TSA treatment for 18hr). B : Control with 2.5 μg of pre-immune rabbit IgG. C : Immunoprecipitation of Histone H3 (acetyl K27) protein by 2.5 μg of Histone H3 (acetyl K27) antibody . 12% SDS-PAGE The immunoprecipitated Histone H3 (acetyl K27) protein was detected by Histone H3 (acetyl K27) antibody diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
Histone H3 (acetyl Lys27) antibody detects Histone H3 (acetyl Lys27) protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 µg HeLa whole cell lysate/extract (0.4 µM Trichostatin A treatment for 18 hr) 15% SDS-PAGE Histone H3 (acetyl Lys27) antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H3K27ac (acetyl Lys27) antibody dilution: 1:500.Application
Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Histone H3K27ac (acetyl Lys27) antibody dilution: 1:500.Application
Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Histone H3K27ac (acetyl Lys27) antibody dilution: 1:500.Application
Dotblot analysis of anti-Histone H3 (acetyl Lys27) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys27) antibody 1:5000 dilution. A: Peptide samples of H3K18 un B: Peptide samples of H3K18ac C: Peptide samples of H3K9un D: Peptide samples of H3K9ac E: Peptide samples of H3K14un F: Peptide samples of H3K14ac G: Peptide samples of H3K27un H: Peptide samples of H3K27acApplication
Histone H3 (acetyl Lys27) antibody detects Histone H3 (acetyl Lys27) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (acetyl Lys27) protein stained by Histone H3 (acetyl Lys27) antibody diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Histone H3K27ac (acetyl Lys27) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (acetyl Lys27) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | acetyl Lys27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | ChimpanzeeChimpanzee (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 1 H3a H3/A HIST1H3A H3FA |
|---|---|
| Target | Histone H3 (acetyl Lys27) |
| Cellular Localization | Nucleus |
Application
Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H3K27ac (acetyl Lys27) antibody. The precipitated DNA was detected by PCR with primer set targeting to RPL10 promoter or β-globin promoter.
Application
Histone H3 (acetyl K27) antibody immunoprecipitates Histone H3 (acetyl K27) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H3 (acetyl K27) protein expressing HeLa cells (0.4μM TSA treatment for 18hr). B : Control with 2.5 μg of pre-immune rabbit IgG. C : Immunoprecipitation of Histone H3 (acetyl K27) protein by 2.5 μg of Histone H3 (acetyl K27) antibody . 12% SDS-PAGE The immunoprecipitated Histone H3 (acetyl K27) protein was detected by Histone H3 (acetyl K27) antibody diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
Histone H3 (acetyl Lys27) antibody detects Histone H3 (acetyl Lys27) protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 µg HeLa whole cell lysate/extract (0.4 µM Trichostatin A treatment for 18 hr) 15% SDS-PAGE Histone H3 (acetyl Lys27) antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H3K27ac (acetyl Lys27) antibody dilution: 1:500.
Application
Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. Histone H3K27ac (acetyl Lys27) antibody dilution: 1:500.
Application
Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Histone H3K27ac (acetyl Lys27) antibody dilution: 1:500.
Application
Dotblot analysis of anti-Histone H3 (acetyl Lys27) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys27) antibody 1:5000 dilution. A: Peptide samples of H3K18 un B: Peptide samples of H3K18ac C: Peptide samples of H3K9un D: Peptide samples of H3K9ac E: Peptide samples of H3K14un F: Peptide samples of H3K14ac G: Peptide samples of H3K27un H: Peptide samples of H3K27ac
Application
Histone H3 (acetyl Lys27) antibody detects Histone H3 (acetyl Lys27) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (acetyl Lys27) protein stained by Histone H3 (acetyl Lys27) antibody diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
