Application
ChIP was performed with HeLa nuclear extract and either 15 μl of H4K8ac antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to ALDOA promoter or MyoD.Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K36me2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (lane 1). Lane 2 shows the same analysis after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature.Application
ICC/IF analysis of HeLa cells using H3K36me2 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K36me2 antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).Application
Dot blot analysis of peptides containing modified and unmodified sequences of histone H3 using H3K36me2 antibody at a dilution of 1:100,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.Application
ELISA was performed using a serial dilution of Histone H3K36me2 (di-Methyl Lys36) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:31,000.| Product Name | Histone H3K36me2 (dimethyl Lys36) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K36me2 (dimethyl Lys36) |
| Modification | dimethyl Lys36 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAICC/IFWBChIP |
| ChIP/ChIP-seq (5 µl/IP), ELISA (1:1,000), Dot blotting (1:100,000), Western blotting (1:1,000), Immunofluoresence (1:500): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Histone H3 di Methyl di Methyl Histone H3 histone cluster 1 H3a H3/A HIST1H3A Histone H3 di-Methyl di-Methyl Lys36 Histone H3 Histone H3 di-Methyl K36 H3FA di Methyl K36 Histone H3 di Methyl Histone H3 Lys36 diMethyl Histone H3 diMethyl Lys36 |
|---|---|
| Target | Histone H3 (di-Methyl Lys36) |
Application
ChIP was performed with HeLa nuclear extract and either 15 μl of H4K8ac antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to ALDOA promoter or MyoD.
Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K36me2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (lane 1). Lane 2 shows the same analysis after incubation of the antibody with 5 nmol blocking peptide for 1 hour at room temperature.
Application
ICC/IF analysis of HeLa cells using H3K36me2 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K36me2 antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
Application
Dot blot analysis of peptides containing modified and unmodified sequences of histone H3 using H3K36me2 antibody at a dilution of 1:100,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.
Application
ELISA was performed using a serial dilution of Histone H3K36me2 (di-Methyl Lys36) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:31,000.| Application Notes | ChIP/ChIP-seq (5 µl/IP), ELISA (1:1,000), Dot blotting (1:100,000), Western blotting (1:1,000), Immunofluoresence (1:500): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
