Application
ChIP was performed with HeLa nuclear extract and either 5 μg of H3K9me1 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to GAPDH or Sat2.Application
Dot blot analysis of peptides containing other histone modifications and the unmodified H3K79 using H3K79me1 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.Application
WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H3 (lane 3) using H3K79me1 antibody diluted 1:200 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of Histone H3K79me1 (mono-Methyl Lys79) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,500.| Product Name | Histone H3K79me1 (monmethyl Lys79) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K79me1 (monmethyl Lys79) |
| Modification | monmethyl Lys79 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAWBChIP |
| ChIP (2 μg/IP), ELISA (1:500), Dot (1:20,000), WB (1:200): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | mono Methyl K79 Histone H3 monoMethyl K79 Histone H3 histone cluster 1 H3a H3/A HIST1H3A mono Methyl Histone H3 Histone H3 monoMethyl H3FA Histone H3 mono-Methyl K79 Histone H3 mono Methyl Methyl Histone H3 mono Methyl Histone H3 K79 mono Meth |
|---|---|
| Target | Histone H3 (mono-Methyl Lys79) |
Application
ChIP was performed with HeLa nuclear extract and either 5 μg of H3K9me1 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to GAPDH or Sat2.
Application
Dot blot analysis of peptides containing other histone modifications and the unmodified H3K79 using H3K79me1 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
Application
WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H3 (lane 3) using H3K79me1 antibody diluted 1:200 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of Histone H3K79me1 (mono-Methyl Lys79) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,500.| Application Notes | ChIP (2 μg/IP), ELISA (1:500), Dot (1:20,000), WB (1:200): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
