Application
Dot blot analysis of peptides containing modifications of histone H3 using H3K79me1 antibody at a dilution of 1:100,000. One hundred to 0.2 pmol of the peptides were spotted on a membrane for analysis.Application
WB analysis of histone extracts from HeLa cells (15 μg) using H3K79me1 antibody at a dilution of 1:1,000 and 1:2000 in TBS-Tween containing 5% skimmed milk.Application
ChIP was performed with HeLa extracts and either control IgG (5 μg) or Histone H3K79me1 (mono-Methyl Lys79) antibody ( 5, 10 and 15 μl per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter and the exon 2 of MB gene.Application
ChIP was performed with HeLa extracts and either control IgG (5 μg) or Histone H3K79me1 (mono-Methyl Lys79) antibody (10 μl). The precipitated DNA was detected by qPCR with primers targeting to RPL30, ALDOA, SERPINA1 and SAA4 genes.Application
ELISA was performed using a serial dilution of Histone H3K79me1 (mono-Methyl Lys79) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.| Product Name | Histone H3K79me1 (monomethyl Lys79) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K79me1 (monomethyl Lys79)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | Polyclonal antibody raised in rabbit against histone H3 the monomethylated lysine 79 (H3K79me1), using a KLH-conjugated synthetic peptide. |
| Modification | monomethyl Lys79 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAWBChIP |
| ChIP (5 – 10 µl/ChIP), ELISA (1:500 – 1:1,000), Dot blotting (1:100,000), Western blotting (1:1,000): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | histone cluster 1 H3a H3/A HIST1H3A H3FA |
|---|---|
| Target | H3 (mono-Methyl Lys79) |
Application
Dot blot analysis of peptides containing modifications of histone H3 using H3K79me1 antibody at a dilution of 1:100,000. One hundred to 0.2 pmol of the peptides were spotted on a membrane for analysis.
Application
WB analysis of histone extracts from HeLa cells (15 μg) using H3K79me1 antibody at a dilution of 1:1,000 and 1:2000 in TBS-Tween containing 5% skimmed milk.
Application
ChIP was performed with HeLa extracts and either control IgG (5 μg) or Histone H3K79me1 (mono-Methyl Lys79) antibody ( 5, 10 and 15 μl per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to GAPDH promoter and the exon 2 of MB gene.
Application
ChIP was performed with HeLa extracts and either control IgG (5 μg) or Histone H3K79me1 (mono-Methyl Lys79) antibody (10 μl). The precipitated DNA was detected by qPCR with primers targeting to RPL30, ALDOA, SERPINA1 and SAA4 genes.
Application
ELISA was performed using a serial dilution of Histone H3K79me1 (mono-Methyl Lys79) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:30,000.| Application Notes | ChIP (5 – 10 µl/ChIP), ELISA (1:500 – 1:1,000), Dot blotting (1:100,000), Western blotting (1:1,000): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
