Application
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.Application
ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5ug). Real-time PCR was performed using primers specific to the gene indicated.Application
RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.| Product Name | Histone H3K79me2 (Dimethyl Lys79) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM181] to Histone H3 (Dimethyl Lys79)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79). |
| Modification | Dimethyl Lys79 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM181 |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWBChIPMultiplexing |
| IHC:1:50~1:200 WB:1:500~1:2000 ChIP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIST1H3A H3/A histone cluster 1 H3a H3FA H3K79Me2 antibody |
|---|---|
| Target | Histone H3 (Dimethyl Lys79) |
| Tissue Specificity | This antibody reacts to Histone H3 dimethylated at Lysine 79 (K79me2). No cross reactivity with monomethylated Lysine 79(K79me1) or trimethylated Lysine 79 (K79me3), or other methylation in histone H3. |
Application
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
Application
ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Application
RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.| Application Notes | IHC:1:50~1:200 WB:1:500~1:2000 ChIP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
