Application
ChIP was performed with HeLaS3 nuclear extract and either 5 μg of H3K79me3 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to GAPDH promoter or myoglobin.Application
Dot blot analysis of peptides containing other histone modifications and the unmodified H3K79 using H3K79me3 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K79me3 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of Histone H3K79me3 (tri-Methyl Lys79) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,500.WB
(B) Assay of the global histone methylation patterns at H3K79 in WT and ΔPodot1 through Western blot. Histone H3 was used as the loading control.| Product Name | Histone H3K79me3 (trimethyl Lys79) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K79me3 (trimethyl Lys79) |
| Modification | trimethyl Lys79 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ChIPDotELISAWB |
| ChIP/ChIP-seq (2 µg/ChIP), ELISA (1:500 – 1:1,000), Dot blotting (1:50,000), Western blotting (1:500): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | tri Methyl Lys79 Histone H3 histone cluster 1 H3a H3/A HIST1H3A tri-Methyl Lys79 Histone H3 Histone H3 tri Methyl triMethyl K79 Histone H3 Histone H3 triMethyl tri Methyl Histone H3 Lys79 H3FA tri-Methyl K79 Histone H3 triMethyl Histone H3 Met |
|---|---|
| Target | Histone H3 (tri-Methyl Lys79) |
Application
ChIP was performed with HeLaS3 nuclear extract and either 5 μg of H3K79me3 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to GAPDH promoter or myoglobin.
Application
Dot blot analysis of peptides containing other histone modifications and the unmodified H3K79 using H3K79me3 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K79me3 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of Histone H3K79me3 (tri-Methyl Lys79) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:3,500.
WB
(B) Assay of the global histone methylation patterns at H3K79 in WT and ΔPodot1 through Western blot. Histone H3 was used as the loading control.| Application Notes | ChIP/ChIP-seq (2 µg/ChIP), ELISA (1:500 – 1:1,000), Dot blotting (1:50,000), Western blotting (1:500): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C/ -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
