Application
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM161 at 0.25 ug/mL, showed a band of histone H3 acetylated at Lysine 9 in treated HeLa.Application
Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161,1 ug/mL) as the detection antibody.Application
ChIP performed on HeLa cells using H3K9ac antibody (RM161, 5ug). Real-time PCR was performed using primers specific to the gene indicated.Application
RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.Application
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys9) Rabbit mAb RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Product Name | Histone H3K9ac (Acetyl Lys9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM161] to Histone H3 (Acetyl Lys9)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9). |
| Modification | Acetyl Lys9 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM161 |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCWBChIPMultiplexing |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 ChIP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HIST1H3A H3/A histone cluster 1 H3a H3FA H3K9Ac antibody |
|---|---|
| Target | Histone H3 (Acetyl Lys9) |
| Tissue Specificity | This antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3. |
Application
Western Blot of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+), using RM161 at 0.25 ug/mL, showed a band of histone H3 acetylated at Lysine 9 in treated HeLa.
Application
Sandwich ELISA against acetylated histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with Sodium Butyrate. Using anti-H3CT (RM188, 1 ug/mL) as the capture antibody and biotinylated anti-H3K9ac (RM161,1 ug/mL) as the detection antibody.
Application
ChIP performed on HeLa cells using H3K9ac antibody (RM161, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Application
RM161 specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.
Application
Immunocytochemistry of HeLa cells treated with sodium butyrate, using Acetyl-Histone H3 (Lys9) Rabbit mAb RM161 (red). Actin filaments have been labeled with fluorescein phalloidin (green).| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 ChIP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS with 50% Glycerol and 1% BSA. 0.09% Sodium azide was added as a preservative. |
