Application
ChIP was performed with U2OS nuclear extract and either 15 μl of H3K9acS10p antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or ALDOA promoter.Application
ELISA was performed using a serial dilution of Histone H3K9acS10ph (acetyl Lys9, phospho Ser10) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:89,000.Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K9acS10ph antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk.Application
Dot blot analysis peptides containing modified histone H4 and H3 or unmodified histone H3 sequences using H3K9acS10ph antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.Application
ICC/IF analysis of HeLa cells using H3K9acS10ph antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10ph antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI.| Product Name | Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K9acS10ph (phospho Ser10, acetyl Lys9) |
| Modification | phospho Ser10, acetyl Lys9 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAICC/IFWBChIP |
| ChIP (15 µl/ChIP), ELISA (1:1,000 – 1:4,000), Dot blotting (1:20,000), Western blotting (1:250), Immunofluoresence (1:500): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | phospho Histone H3 (pSer10) histone cluster 1 H3a acetyl Histone H3 H3/A HIST1H3A phospho Ser10 acetyl Lys9 Histone H3 phospho Histone H3 (pS10) Histone H3 phospho Ser10 acetyl Lys9 Histone H3 (phospho S10) phospho S10 acetyl K9 Histone H3 Hi |
|---|---|
| Target | Histone H3 (acetyl Lys9, phospho Ser10) |
Application
ChIP was performed with U2OS nuclear extract and either 15 μl of H3K9acS10p antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or ALDOA promoter.
Application
ELISA was performed using a serial dilution of Histone H3K9acS10ph (acetyl Lys9, phospho Ser10) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:89,000.
Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K9acS10ph antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk.
Application
Dot blot analysis peptides containing modified histone H4 and H3 or unmodified histone H3 sequences using H3K9acS10ph antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.
Application
ICC/IF analysis of HeLa cells using H3K9acS10ph antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9acS10ph antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI.| Application Notes | ChIP (15 µl/ChIP), ELISA (1:1,000 – 1:4,000), Dot blotting (1:20,000), Western blotting (1:250), Immunofluoresence (1:500): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
