Application
ChIP was performed with U2OS nuclear extract and either 10 μl of H3K9me1 antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH promoter.Application
Dot blot analysis of peptides containing modified histone H3 using H3K9me1 antibody at a dilution of 1:200,000. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane for analysis.Application
ICC/IF analysis of HeLa cells using H3K9me1 antibody and DAPI. Cells were formaldehyde fixated, permeabilized with Triton X-100 and blocked with PBS containing 2.5% BSA. The cells were immunofluorescently labelled with the H3K9me1 antibody at a dilution of 1:200 (green). The nuclei were stained with DAPI.Application
WB analysis of histone extracts (15 μg) of HeLa cells using H3K9me1 antibody diluted 1:1000 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of Histone H3K9me1 (mono-Methyl Lys9) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:50,000.| Product Name | Histone H3K9me1 (monomethyl Lys9) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K9me1 (monomethyl Lys9) |
| Modification | monomethyl Lys9 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAICC/IFWBChIP |
| ChIP (10 µl/ChIP), ELISA (1:2,000 – 1:3,000), Dot blotting (1:200,000), Western blotting (1:1000), Immunofluorescence (1:200): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | mono Methyl Lys9 Histone H3 mono-Methyl K9 Histone H3 histone cluster 1 H3a H3/A HIST1H3A mono Methyl K9 Histone H3 mono Methyl Histone H3 Histone H3 monoMethyl monoMethyl Lys9 Histone H3 H3FA Histone H3 mono Methyl monoMethyl K9 Histone H3 Me |
|---|---|
| Target | Histone H3 (mono-Methyl Lys9) |
Application
ChIP was performed with U2OS nuclear extract and either 10 μl of H3K9me1 antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH promoter.
Application
Dot blot analysis of peptides containing modified histone H3 using H3K9me1 antibody at a dilution of 1:200,000. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane for analysis.
Application
ICC/IF analysis of HeLa cells using H3K9me1 antibody and DAPI. Cells were formaldehyde fixated, permeabilized with Triton X-100 and blocked with PBS containing 2.5% BSA. The cells were immunofluorescently labelled with the H3K9me1 antibody at a dilution of 1:200 (green). The nuclei were stained with DAPI.
Application
WB analysis of histone extracts (15 μg) of HeLa cells using H3K9me1 antibody diluted 1:1000 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of Histone H3K9me1 (mono-Methyl Lys9) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:50,000.| Application Notes | ChIP (10 µl/ChIP), ELISA (1:2,000 – 1:3,000), Dot blotting (1:200,000), Western blotting (1:1000), Immunofluorescence (1:200): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
