Application
ChIP was performed with HeLa nuclear extract and either 2 μg of H3K9me2 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH promoter.Application
Dot blot analysis of peptides containing modified histone H3 and H4 and the unmodified H3K9 sequence using H3K9me2 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane for analysis.Application
ICC/IF analysis of NIH3T3 cells using H3K9me2 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI.Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K9me2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of Histone H3K9me2 (di-Methyl Lys9) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.| Product Name | Histone H3K9me2 (dimethyl Lys9) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K9me2 (dimethyl Lys9) |
| Modification | dimethyl Lys9 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAICC/IFWBChIP |
| ChIP (2 µg/ChIP), ELISA (1:1,000), Dot blotting (1:20,000), Western blotting (1:1,000), Immunofluorescence (1:500): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | di-Methyl Lys9 Histone H3 Histone H3 di-Methyl Lys9 Histone H3 di-Methyl K9 Histone H3 di Methyl di Methyl K9 Histone H3 di-Methyl K9 Histone H3 di Methyl Histone H3 histone cluster 1 H3a H3/A HIST1H3A Histone H3 di-Methyl H3FA diMethyl Histon |
|---|---|
| Target | Histone H3 (di-Methyl Lys9) |
Application
ChIP was performed with HeLa nuclear extract and either 2 μg of H3K9me2 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH promoter.
Application
Dot blot analysis of peptides containing modified histone H3 and H4 and the unmodified H3K9 sequence using H3K9me2 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane for analysis.
Application
ICC/IF analysis of NIH3T3 cells using H3K9me2 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K9me2 antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI.
Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K9me2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of Histone H3K9me2 (di-Methyl Lys9) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:103,000.| Application Notes | ChIP (2 µg/ChIP), ELISA (1:1,000), Dot blotting (1:20,000), Western blotting (1:1,000), Immunofluorescence (1:500): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
