Application
ChIP was performed with U2OS nuclear extract and either 15 μl of H3R17me2 (asym) antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to Myoglobin or GAPDH promoter.Application
Dot blot analysis of peptides containing modified histone H3 and H4 and unmodified sequences from histone H3 using H3R17me2(asym) antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3R17me2(asym) antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of Histone H3R17me2 (di-Methyl Arg17) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.| Product Name | Histone H3R17me2 (Asymmetric dimethyl Arg17) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3R17me2 (dimethyl Arg17) |
| Modification | Asymmetric dimethyl Arg17 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAWBChIP |
| ChIP (10 - 15 µl/ChIP), ELISA (1:1,000 – 1:3,000), Dot blotting (1:20,000): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Histone H3 di Methyl diMethyl Arg17 Histone H3 Histone H3 di-Methyl Arg17 di Methyl Histone H3 histone cluster 1 H3a H3/A HIST1H3A di Methyl Histone H3 Arg17 Histone H3 di-Methyl di Methyl Histone H3 R17 di Methyl Arg17 Histone H3 diMethyl R17 |
|---|---|
| Target | Histone H3 (di-Methyl Arg17) |
Application
ChIP was performed with U2OS nuclear extract and either 15 μl of H3R17me2 (asym) antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to Myoglobin or GAPDH promoter.
Application
Dot blot analysis of peptides containing modified histone H3 and H4 and unmodified sequences from histone H3 using H3R17me2(asym) antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.
Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3R17me2(asym) antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of Histone H3R17me2 (di-Methyl Arg17) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:40,000.| Application Notes | ChIP (10 - 15 µl/ChIP), ELISA (1:1,000 – 1:3,000), Dot blotting (1:20,000): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
