Application
Dotblot analysis of anti-Histone H3 (phospho Ser28) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (phospho Ser28) antibody 1:5000 dilution. A: Peptide samples of H3S10 un B: Peptide samples of H3S10ph C: Peptide samples of H3S28 un D: Peptide samples of H3S28phApplication
Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at nucleus on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Histone H3 (phospho Ser28) antibody dilution: 1:500.Application
Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Histone H3 (phospho Ser28) antibody dilution: 1:500.Application
Histone H3 (phospho S28) antibody detects Histone H3 (phospho S28) protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho S28) antibody dilution: 1:1000Application
Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at chromosome in assynchronous HeLa cells. Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min. Green: Histone H3 (phospho Ser28) protein stained by Histone H3 (phospho Ser28) antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Histone H3S28ph (phospho Ser28) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (phospho S28) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | p-Ser28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 1 H3a H3/A HIST1H3A H3FA |
|---|---|
| Target | Histone H3 (phospho Ser28) |
| Cellular Localization | Nucleus |
Application
Dotblot analysis of anti-Histone H3 (phospho Ser28) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (phospho Ser28) antibody 1:5000 dilution. A: Peptide samples of H3S10 un B: Peptide samples of H3S10ph C: Peptide samples of H3S28 un D: Peptide samples of H3S28ph
Application
Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at nucleus on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Histone H3 (phospho Ser28) antibody dilution: 1:500.
Application
Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Histone H3 (phospho Ser28) antibody dilution: 1:500.
Application
Histone H3 (phospho S28) antibody detects Histone H3 (phospho S28) protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho S28) antibody dilution: 1:1000
Application
Histone H3 (phospho Ser28) antibody detects Histone H3 (phospho Ser28) protein at chromosome in assynchronous HeLa cells. Sample: HeLa cells were fixed in 2% paraformaldehyde for 30 min. Green: Histone H3 (phospho Ser28) protein stained by Histone H3 (phospho Ser28) antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HeLa (100ng/ml Nocodazole treatment for 24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
